Creating centimeter-scale complex tissue geometries within seconds via Volumetric Bioprinting

Our finite ability to spontaneously regenerate our organs, associated with increasing longevity, reinforces the need for engineered human tissues. Bioprinting made constructing architecturally complex, centimeter-scale 3D living structures possible in hours, arranging cells and materials into pattern that can hasten maturation into functional tissues. Nevertheless, biological elements such as cells and biomolecules are sensitive to physical stimuli and have shortened lifetime in solution. This can result in the reduction of cell function proportionally to printing time.

Volumetric Bioprinting is a revolutionary technology that produces tissue constructs of virtually any geometry within seconds through tomographic visible light projections. As a result, it is possible to fabricate free-form and hollow structures with minimal cell distress. We employed this technology to build up multi-material constructs that mimic human organs of layered tissues, such as the bone marrow, and to the creation of perfusable metabolic biofactories based on liver-cell organoids. While the technology is still in its infancy, new advances in Volumetric Bioprinting can open exciting new possibilities for the fabrication of human tissues and their application in regenerative medicine.

Presented by Marisa Assunção, Postdoctoral researcher at Regenerative Medicine Center Utrecht, Dept. of Orthopaedics, University Medical Center Utrecht.

About Marisa Assunção
Marisa Assunção obtained a Ph.D. in Biomedical Sciences from the Chinese University of Hong Kong comprising the engineering of cell-derived extracellular matrix-based biomaterials for clinical application (patent pending). Before, she obtained an MSc in Molecular Genetics from University of Minho, and a first-class honours BSc(Hons) in Pathological Anatomy from Instituto Politécnico de Saúde-Norte, Portugal. Other contributions in Regenerative Medicine include R&D of nucleic acid-loaded nanoparticles (INEB, Portugal) and vascular patches (HZG, Germany). Her postdoctoral research at University Medical Center Utrecht (UMCU) focuses on applying volumetric bioprinting to organotypic model engineering.

About Regenerative Medicine Center Utrecht, Dept. of Orthopaedics, University Medical Center Utrecht
Within the Regenerative Medicine Center Utrecht and University Medical Center Utrecht, Our lab, lead by Prof. Riccardo Levato focuses on the development of Biofabrication strategies to create bioprinted and lab-made tissue models, particularly for musculoskeletal regeneration. Prof. Levato’s lab also focuses on the design of novel biofabrication technologies, particularly in relation to light-based bioprinting, such as Volumetric Bioprinting.

Marisa Assunção is speaker at the 2022 edition of the 3DBioprinting Conference.

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